An endometrial biopsy is a procedure performed to obtain a small tissue sample from the lining of the uterus, called the endometrium. After the biopsy, the endometrial tissue is examined under a microscope to identify the presence of abnormal cells, or the effects of hormones on the endometrium.
Other related procedures used to evaluate and treat endometrial problems include dilation and curettage (D & C), hysteroscopy, and endometrial ablation. Please see these procedures for additional information.
With each menstrual cycle, the endometrium prepares itself to nourish a fetus, as increased levels of estrogen and progesterone help to thicken its walls. If implantation of the fertilized egg does not occur, the endometrium, coupled with blood and mucus from the vagina and cervix (the lower, narrow part of the uterus located between the bladder and the rectum) make up the menstrual flow (also called menses) that leaves the body through the vagina. After menopause, menstruation stops and a woman should not have any bleeding.
The organs and structures of the female pelvis are:
Endometrium. This is he lining of the uterus.
Uterus (also called the womb). The uterus is a hollow, pear-shaped organ located in a woman's lower abdomen, between the bladder and the rectum. The uterus sheds its lining each month during menstruation, unless a fertilized egg (ovum) becomes implanted and pregnancy follows.
Ovaries. Two female reproductive organs located in the pelvis in which egg cells (ova) develop and are stored and where the female sex hormones estrogen and progesterone are produced.
Cervix. The lower, narrow part of the uterus located between the bladder and the rectum, forming a canal that opens into the vagina, which leads to the outside of the body.
Vagina (also called the birth canal). The passageway through which fluid passes out of the body during menstrual periods. The vagina connects the cervix and the vulva (the external genitalia).
Vulva. The external portion of the female genital organs.
Fallopian tube. Two thin tubes that extend from each side of the uterus, toward the ovaries, as a passageway for eggs and sperm.
An endometrial biopsy may be recommended for women with abnormal menstrual bleeding, bleeding after menopause, or absence of uterine bleeding. Biopsy results may indicate cell changes related to hormone levels, or the presence of abnormal tissues, such as fibroids or polyps, which can lead to abnormal bleeding. Endometrial biopsy may also be used to check for uterine infections, such as endometritis.
An endometrial biopsy may also be used to check the effects of hormone therapy or to detect the presence of abnormal cells or cancer. Endometrial cancer is the most common cancer of the female reproductive organs. Endometrial biopsy is no longer recommended as a routine part of the evaluation and treatment of infertility (inability to conceive).
There may be other reasons for your health care provider to recommend an endometrial biopsy.
As with any surgical procedure, complications may occur. Some possible complications may include, but are not limited to, the following:
Puncture of the uterine wall with the biopsy device, which is rare
Patients who are allergic to or sensitive to medications, iodine, or latex should notify their health care provider.
If you are pregnant or suspect that you may be pregnant, you should notify your health care provider. Endometrial biopsy during pregnancy may lead to miscarriage.
There may be other risks depending on your specific medical condition. Be sure to discuss any concerns with your health care provider prior to the procedure.
Certain factors or conditions may interfere with an endometrial biopsy. These factors include, but are not limited to, the following:
Acute vaginal or cervical infections
Acute pelvic inflammatory disease
Your health care provider will explain the procedure to you and offer you the opportunity to ask any questions that you might have about the procedure.
You will be asked to sign a consent form that gives your permission to do the procedure. Read the form carefully and ask questions if something is not clear.
Generally, no prior preparation, such as fasting or sedation, is required. However, your health care provider may recommend that you take a pain reliever 30 minutes before the procedure.
If you are pregnant or suspect that you are pregnant, you should notify your health care provider.
Notify your health care provider if you are sensitive to or are allergic to any medications, iodine, latex, tape, and anesthetic agents (local and general).
Notify your health care provider of all medications (prescription and over-the-counter) and herbal supplements that you are taking.
Notify your health care provider if you have a history of bleeding disorders or if you are taking any anticoagulant (blood-thinning) medications, aspirin, or other medications that affect blood clotting. It may be necessary for you to stop these medications prior to the procedure.
Your health care provider may request that you keep a record of your menstrual cycles. You may need to schedule the procedure for a specific time of your cycle.
If a sedative is given before the procedure, you will need someone to drive you home afterwards.
You may want to bring a sanitary napkin to wear home after the procedure.
Based on your medical condition, your doctor may request other specific preparation.
An endometrial biopsy may be performed in a doctor’s office, on an outpatient basis, or as part of your stay in a hospital. Procedures may vary depending on your condition and your health care provider’s practices.
Generally, an endometrial biopsy follows this process:
You will be asked to undress completely or from the waist down and put on a hospital gown.
You will be instructed to empty your bladder prior to the procedure.
You will lie on an examination table, with your feet and legs supported as for a pelvic examination.
Your health care provider will insert an instrument called a speculum into your vagina to spread the walls of the vagina apart to expose the cervix.
Your cervix will be cleansed with an antiseptic solution.
The health care provider may numb the area using a small needle to inject medication, or a numbing spray may be applied to your cervix.
A type of forceps, called a tenaculum, may be used to hold the cervix steady for the biopsy. You may feel some cramping when the tenaculum is applied.
A thin, rod-like instrument, called a uterine sound, may be inserted through the cervical opening to determine the length of the uterus and location for biopsy. This may cause some cramping. The sound will then be removed.
A thin tube, called a catheter, will be inserted through the cervical opening into the uterus. The catheter has a smaller tube (internal piston) inside it. The health care provider will withdraw the inner tube creating suction at the end of the catheter. The health care provider will then gently rotate and move the tip of the catheter in and out to collect small pieces of endometrial tissue. This may cause some cramping.
The amount and location of tissue removed depends on the reason for the endometrial biopsy.
The catheter and speculum will be removed and the tissue will be placed in a preservative.
The endometrial tissue will be sent to a lab for examination.
After the procedure, you may rest for a few minutes before going home. If you received any type of sedative, you will need someone to drive you home.
You may want to wear a sanitary pad for bleeding. It is normal to have some mild cramping and spotting or vaginal bleeding for a few days after the procedure. Take a pain reliever for soreness as recommended by your health care provider. Aspirin or certain other pain medications may increase the chance of bleeding. Be sure to take only recommended medications.
You may be instructed not to douche, use tampons, or have intercourse for two to three days after an endometrial biopsy, or for a period of time recommended by your health care provider.
You may also have other restrictions on your activity, including no strenuous activity or heavy lifting.
You may resume your normal diet unless your health care provider advises you differently.
Your health care provider will advise you on when to return for further treatment or care.
Notify your health care provider if you have any of the following:
Excessive bleeding, or bleeding longer than two days after the procedure
Foul-smelling drainage from your vagina
Fever and/or chills
Severe lower abdominal pain
Your health care provider may give you additional or alternate instructions after the procedure, depending on your particular situation.
The content provided here is for informational purposes only, and was not designed to diagnose or treat a health problem or disease, or replace the professional medical advice you receive from your health care provider. Please consult your health care provider with any questions or concerns you may have regarding your condition.
This page contains links to other websites with information about this procedure and related health conditions. We hope you find these sites helpful, but please remember we do not control or endorse the information presented on these websites, nor do these sites endorse the information contained here.
American Cancer Society
American College of Obstetricians and Gynecologists
National Cancer Institute (NCI)
National Institutes of Health (NIH)
National Library of Medicine
National Women's Health Information Center